Flow-through tyrosinase enzyme reactor based on reticulated vitreous carbon functionalized by an electrochemically synthesized film

The use of electrochemically synthesized polymers to modify the surface of reticulated vitreous carbon (RVC) electrodes, introducing functional groups useful for a covalent linking of enzymes, is described. Tyrosinase was immobilized by glutaraldehyde crosslinking over a poly(tyramine) modified RVC. The usefulness of such an approach is demonstrated by the fabrication of a RVC flow-through tyrosinase reactor.