Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application

Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml−1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.