Title of article
Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application
Author/Authors
H. Yanagihara، نويسنده , , R. Sakata، نويسنده , , H. MINAMI، نويسنده , , H. Tanaka، نويسنده , , Y. Shoyama، نويسنده , , H. Murakami، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1996
Pages
8
From page
63
To page
70
Abstract
Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml−1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.
Keywords
Purification , Chromatography , MAB , Forskolin , ELISA , Affinity column
Journal title
Analytica Chimica Acta
Serial Year
1996
Journal title
Analytica Chimica Acta
Record number
1024331
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