Mediator type of glucose microbial biosensor based on Aspergillus niger

Whole cells of Aspergillus niger CCM 8004 containing glucose oxidase (EC 1.1.3.4.) were used for the construction of an amperometric microbial mediated carbon paste biosensor. The microorganism was either placed on the surface of the electrode or incorporated directly into the carbon paste. The mediators were either dissolved in the buffer (hexacyanoferrate(III) and ferrocene) or loaded in the carbon paste (ferrocene). All methods resulted in effective glucose amperometric biosensors. The operational stability of the surface-layer modified whole cell biosensor based on ferrocene incorporated into the carbon paste was at least one month, the upper linearity limit was 6 mM. The sensor was used for measuring the glucose content in real samples.