Modeling and optimization studies for a sequential flow based bio-analytical system

The clinical applications of the sequential flow technique are emphasized in this study by considering glucose determinations in a standard blood chemistry analysis. The sequential flow configuration is modeled to predict experimental behavior. A rigorous theoretical formulation of the model is presented incorporating temperature relationships for the hydrodynamic and kinetic parameters. A comparisons of model predictions with experiments show agreement satisfactory for determining optimal design strategies. To perform this analysis, factorial design results of model predictions are used to locate regions where a full set of model predictions should be made. Also, experimental results and theoretical predictions are used to determine the best temperature for glucose determinations.