Title of article
Sequence-specific electrochemical biosensing of M. tuberculosis DNA
Author/Authors
Joseph Wang، نويسنده , , Gustavo Rivas، نويسنده , , Xiaohua Cai، نويسنده , , Narasaiah Dontha، نويسنده , , Haruki Shiraishi، نويسنده , , Denbai Luo، نويسنده , , Florenda S. Valera، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1997
Pages
8
From page
41
To page
48
Abstract
An electrochemical biosensor for the determination of short sequences from the Mycobacterium tuberculosis (MTB) DNA is described. The sensor relies on the modification of the carbon-paste transducer with 27- or 36-mer oligonucleotide probes and their hybridization to complementary strands from the MTB DNA direct repeat region. Chronopotentiometry is employed to transduce the hybridization event, in connection with a Co(phen)33+ indicator. Short (5–15 min) hybridization periods permit convenient quantitation of ng ml−1 levels of the MTB DNA target. Similar results are observed using microfabricated carbon-strip transducers. The new sensor holds great promise for rapid screening for MTB infections. Direct adsorptive stripping potentiometric measurements of ultratrace levels of MTB DNA are also reported.
Keywords
Biosensors , M. tuberculosis , Sequence-specific detection , Hybridization , Carbon-paste electrode , Screen-printed electrode , DNA
Journal title
Analytica Chimica Acta
Serial Year
1997
Journal title
Analytica Chimica Acta
Record number
1025188
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