• Title of article

    Highly sensitive detection of membrane-bound DNA using fluorescein derivatives

  • Author/Authors

    Satoshi Fujita، نويسنده , , Takeshi Toru، نويسنده , , Yukiko Kitagawa، نويسنده , , Naoto Kagiyama، نويسنده , , Masayoshi Momiyama، نويسنده ,

  • Issue Information
    روزنامه با شماره پیاپی سال 1997
  • Pages
    8
  • From page
    289
  • To page
    296
  • Abstract
    A number of fluorescein derivatives were synthesized and their fluorescent properties were studied. Diphosphates of fluorescein derivatives were tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound DNA to give high resolution detection of DNA. DNA was found to be detectable down to 20 fg in an assay using N-(2-biphenylcarbonyl)-and N(4-biphenylcarbonyl)-5-aminofluorescein diphosphate.
  • Keywords
    Fluorimetry , DNA , Fluorescein derivatives
  • Journal title
    Analytica Chimica Acta
  • Serial Year
    1997
  • Journal title
    Analytica Chimica Acta
  • Record number

    1025243