Author/Authors :
?tefan Mes?ro?، نويسنده , , ?aneta Va?kov?، نويسنده , , Saul Grunfeld، نويسنده , , Adriana Mes?ro?ov?، نويسنده , , Tadeusz Malinski، نويسنده ,
Abstract :
An amperometric enzyme electrode for superoxide determination was prepared by anodic polymerization of pyrrole and concomitant incorporation of superoxide dismutase on a Pt wire in phosphate buffer solution. The amperometric response to superoxide was measured at a potential of 0.7 V to oxidize the hydrogen peroxide generated. The response time of biosensor was <5 s and a 15 nM detection limit was achieved.