Voltammetric determination of mitomycin C in the presence of other anti-cancer drugs and in urine

Stripping voltammetry (SV) in combination with the hanging mercury drop electrode is used to determine the antineoplastic drug mitomycin C (MMC) alone and in mixtures with 5-fluorouracil and cisplatin. At short accumulation times MMC can be determined at submicromolar concentrations in excess of the above drugs. If the accumulation is performed at the potentials of MMC reduction (−0.35 V vs. SCE), reduced MMC is so strongly adsorbed at the electrode that it is possible to prepare a MMC-modified electrode and transfer it to the background electrolyte to determine MMC by voltammetry. This procedure (transfer stripping voltammetry, TSV) helps to reduce interferences of other substances and can be applied for a direct determination of MMC alone or in its mixtures with other drugs in urine. The determination is very fast and does not require any cleaning up of the urine sample prior the TSV analysis.