Author/Authors :
Ardalan، T. نويسنده Young Researcher Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran, , , Ardalan، P. نويسنده Young Researcher Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran, , , Heravi، M. M. نويسنده Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran ,
Abstract :
Saffron petal is the main by-product of saffron processing which is produced in large amounts, annually. The objectives of this study were to study the antioxidant activity and free radical-scavenging effects of saffron petal extracts. The ability of saffron petal to act as an antioxidant using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free-radical method was investigated by applying the Uv–Vis spectrometry. The Uv–Vis spectra of reaction mixtures in acetonitrile revealed that saffron petal has a considerable effect on scavenging free radical. Kinetic studies were conducted by measuring the disappearance of DPPH in acetonitrile over the wavelength range of 515-522 nm under pseudo-first-order conditions at 37oC. Furthermore, the pseudo first order rate constants were determined.
