Author/Authors :
Ajami، Abolghasem نويسنده Obstetrics and Gynecology Department, Infertility Ward, Emam Khomeini Hospital, Mazandaran University of Medical Science, Sari, Iran , , Shadman، Mojtaba نويسنده Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Shadman, Mojtaba , Rafiei، Alireza نويسنده , , Hosseini، Vahid نويسنده , , Talebi-BezminAbadi، Amin نويسنده Molecular and cell biology cente , Faculty of medicine, Mazandaran University of Medical Sciences, Sari, Iran Talebi-BezminAbadi, Amin , Alizadeh ، Ahad نويسنده Department of Biostatistics, Faculty of Health, Tehran University of Medical Sciences, Tehran, Iran Alizadeh , Ahad , Hosseini-khah، Zahra نويسنده Molecular and cell biology cente , Faculty of medicine, Mazandaran University of Medical Sciences, Sari, Iran Hosseini-khah, Zahra
Abstract :
BACKGROUND AND AIM: Cytotoxin-associated gene A (CagA)-positive
strains of Helicobacter pylori are associated with gastroduodenal diseases.
Evidences have suggested that the type of H. pylori CagA EPIYA motifs may be
associated with recurrent dyspepsia (i.e. gastritis, peptic ulcer, or gastric cancer).
We investigated the prevalence of different EPIYA motifs (A, B, C, or D) in
H. pylori strains isolated from patients with recurrent dyspepsia who underwent
upper gastrointestinal (GI) endoscopy.
We investigated the prevalence of different EPIYA motifs (A, B, C, or D) in H.
pylori strains isolated from patients with recurrent dyspepsia who underwent
upper gastrointestinal (GI) endoscopy.
METHODS: H. pylori strains were isolated from biopsy specimens of 220
patients with recurrent dyspepsia. The presence of glmM gene, as a housekeeping
gene, CagA gene, and pattern of CagA EPIYA motifs were determined using
polymerase chain reaction (PCR) method. The association between the type of
motifs and disease state was determined by the Chi-square test, Fisher’s exact test,
and logistic regression.
RESULTS: CagA-positive H. pylori strains were identified in 125 (57%) of
patients, including 36 (28.6%) gastritis, 31 (24.6%) duodenal ulcer, and 58
(46.4%) gastric cancer. The frequency of pattern of CagA EPIYA motifs were
detected as 39 (31.2%) AB motifs, 54 (43.2%) ABC motifs, 32 (25.6%) ABCC
motifs,and no D motifs. The risk of gastric cancer occurrence was estimated to be
2.57 times higher in patients infected by strains with ABCC motif when compared
with gastritis and duodenal ulcer patients (p=0.03). Moreover, patients with
C-containing motifs were 2.27 times more likely to be afflicted with gastric cancer
than with duodenal ulcer. AB motif was more associated with gastritis and
duodenal ulcer than ABC and ABCC motifs.
CONCLUSION: The results suggested that CagA-EPIYA ABCC might be
associated with gastric cancer, while EPIYA-AB might be associated with
duodenal ulcer.
