γ-Aminobutyrohydroxamate resins as stationary phases of chelation ion chromatography Original Research Article

γ-Aminobutyrohydroxamate resin and its derivatives were prepared and employed as stationary phases in chelation ion chromatography for the simultaneous determination of trace metal ions in sea water and biological sample. The method consists of preconcentration of a 25 ml sea water or biological sample on a γ-aminobutyrohydroxamate resin column; alkali and alkaline earth metal ions are removed from the resin with potassium nitrate (0.01 M) solution, the metal ions are eluted with oxalic acid (0.03 M, pH 2.6) and separated on a Dionex CS-5 column , followed by postcolumn derivatization with 4(2-pyridylazo)resorcinol and spectrophotometric detection at 495 nm. The concentration and separation steps are automated. The method shows good linearity for Co(II), Cu(II), Mn(II), Ni(II) and Zn(II) ranging from 0.5 to 10 ng ml−1 and that for Cd(II) ranging from 2 to 40 ng ml−1. The detection limits for Co(II), Cd(II), Cu(II), Mn(II), Ni(II) and Zn(II) are 0.4, 1.2, 0.5, 0.4, 0.8 and 0.3 ng ml−1, respectively. The analytical reliability of the proposed method was evaluated by analyzing oyster tissue standard reference material (NIST, SRM 1566a).