Application of polyacrylamide slab gel electrophoresis to the analysis and small-scale purification of amyloid proteins Review Article

Polyacrylamide slab gel electrophoresis (PAGE) is widely used in different fields of amyloid research. We review the uses of PAGE for the determination of molecular mass and degree of purity of isolated amyloid proteins, for their immunochemical identification by immunoblotting, and for their chemical characterization using microsequencing strategies. The review demonstrates the role of PAGE in the studies of the processing of amyloid precursor proteins, describes the application of PAGE for detection and typing of amyloid proteins in small biopsy tissues, and discusses the utility of PAGE for small-scale purification of amyloid proteins.