Open tubular capillary electrochromatography with adsorbed stationary phase Original Research Article

A novel preparation method of stationary phase was presented for open tubular capillary electrochromatography (OTCEC). The adsorbed stationary phases were prepared easily by rinse the capillary with a buffer containing cationic surfactant such as cetyltrimethylammonium bromide (CTAB) or basic protein such as lysozyme. After a surfactant bilayer or a protein layer was adsorbed on the capillary wall, the capillary was rinsed again with a running buffer containing no CTAB or protein. The CTAB bilayer was used as a stationary phase for separation of neutral solutes. Five alkyl substituted benzenes were baseline separated within 6 min with theoretical plate number of 223 000/m for benzene and 181 000/m for toluene. The adsorbed lysozyme layer was used as chiral stationary phase for separation of enantiomers. Four amino acids and the drug of mephenytoin were separated within 5 min with a resolution from 1.74 to 2.05.