Instrumentation, standards, and procedures used at the National Research Council of Canada for high-accuracy fluorescence measurements Original Research Article

This paper describes the two-monochromator reference spectrofluorimeter, calibration standards, and procedures used at the National Research Council of Canada for performing high-accuracy fluorescence measurements over the spectral range 250–1050 nm. The basic measurement principles of the two-monochromator method and the calibration procedures used to determine the instrument spectral characteristics are described in detail. The instrumental set-ups, data acquisition procedures, and representative results for the measurement of reflected and bispectral luminescent radiance factors and calculation of total radiance factors are graphically illustrated in a step-by-step fashion for a typical red fluorescent material. The measurement performance of this instrument is demonstrated for several applications, including customer-supplied source data, paper, paint and textile samples.