DNA-modified electrodes Part 3.: spectroscopic characterization of DNA-modified gold electrodes Original Research Article

DNA-modified gold electrodes were characterized by scanning tunneling microscopy (STM), Raman spectroscopy, in situ UV/Vis reflection spectroscopy, X-ray photoelectron spectroscopy (XPS) and alternating current (AC) impedance. It has been found that dsDNA adsorbed firmly on gold surfaces lies strand-on in an ordered saturated monolayer, and ssDNA strands exist in a honeycomb-like form on the surfaces. The bases and phosphate groups of DNA backbone interacting with gold electrode surfaces play an important role in DNA immobilization onto gold electrode surfaces.