Enzyme immunoassay by capillary electrophoresis with laser induced fluorescence detection Original Research Article

Antibody (Ab) labeled with alkaline phosphatase was separated and detected by a capillary electrophoresis system equipped with a laser induced fluorescence detection system. The combination of B/F separation and the LIF detection of the label enzyme is potentially useful for a highly sensitive detection of antigen (Ag). After B/F separation by capillary electrophoresis (CE), label enzymes catalyze the hydrolysis of fluorescein diphosphate in the running buffer for several minutes, producing fluorescein. Experiments with various ratios of Ab to Ag revealed that the faster peak was the complex of Ab and Ag, and the other was free Ab.