Liquid chromatography and exchange reaction of a synthetic thiurams mixture Original Research Article

Reversed-phase liquid chromatography of thiuram monosulphide and disulphides (e.g. thiram, disulfiram) was performed using UV or mass spectrometry detection. Mixed thiuram formation was studied as a function of pH, and methanol/water ratio in the sample solvent. Conditions allowing a largely decreasing exchange reaction rate were based on the use of a 85/15 methanol/water pH 2.5 as sample solvent in vials with septum.