Reversible oligonucleotide immobilisation based on biotinylated polypyrrole film

In this paper, we describe the design of a new versatile and reversible DNA sensor. The system is based on biotin grafting-units, covalently linked to a polypyrrole matrix, able to anchor large biomolecules, thanks to biotin/avidin affinity. These grafting-units can be regenerated after the “denaturation” of the biotin/avidin link allowing to reuse the matrix for the immobilisation of an other assembly and then the possibility to generate a new sensor.