On-line simultaneous determination of S- and R-perindopril using amperometric biosensors as detectors in flow systems Original Research Article

Two types of flow systems were selected for the simultaneous assay of S- and R-perindopril (pdp): flow injection analysis (FIA) and sequential injection analysis (SIA). The SIA system was more efficient, because of the highest precision and accuracy, and the lower consumption of sample and buffer. The amperometric biosensors used as detectors in the flow systems were based on l- and d-amino acid oxidase (AAOD). The linear concentration ranges are in the nmol l−1 range, from 120 pmol l−1 to 40 nmol l−1 (3×S.D.), with very low detection limits. The biosensors/flow system can be used reliably on-line in synthesis process control, for the simultaneous assay of S- and R-pdp with a frequency of more than 30 samples per hour.