Screen-printed electrode microarray for electrochemiluminescent measurements Original Research Article

A microarray of nine screen-printed (SP) graphite electrodes was used to develop multi-parametric electrochemiluminescent (ECL) biochip. The whole biochip is based on the ECL detection of enzymatically generated H2O2. The intrinsic performances of this electrode array were evaluated through the cyclic-voltammetric experiments in 0.1 M ferricyanide. The variations of the specific behaviours (oxidation potential and current) of such a redox mediator were then used to reach the reproducibility of the SP arrays, i.e. 2% for electrode-to-electrode and 4% for biochip-to-biochip variations. In a similar way, electrochemiluminescent cyclic-voltammetric experiments were performed to characterize the ECL reaction triggered at the surface of a SP carbon electrode array. An optimum luminol oxidation potential of +650 mV versus platinum pseudo-reference was found with a reproducibility of 4.4%.