Prussian blue-based optical glucose biosensor in flow-injection analysis Original Research Article

A novel optical biosensor for glucose and its analytical applications are demonstrated. The biosensing part of the flow-through sensor is composed of Prussian blue (PB) film with chemically linked molecules of glucose oxidase (GOx). The utilized biosensing scheme is similar to this reported for oxidase-based first-generation amperometric biosensors. Demonstrated biosensing system exhibits excellent operational (over 1 month) and storage (at least 3 months) stability. Developed flow-injection systems based on the PB/GOx-optical biosensor allow frequent measurements (15 samples/h) in sub-millimolar range of glucose concentration (linear responses from 0.05 to 2.0 mmol/l). Double-channel flow-injection analysis (FIA)/biosensor system has been successfully used in pharmaceutical, food and clinical analysis of real samples containing glucose.