A cloud point extraction approach using Triton X-100 for the separation and preconcentration of Sudan dyes in chilli powder Original Research Article

A CPE-HPLC (UV) method was developed for the determination of Sudan (I–IV) dyes, non-ionic surfactant Triton X-100 was used to extract and preconcentrate Sudan dyes from chilli powder prior to their determination by HPLC-UV. The separation and determination of Sudan dyes was then carried out in the HPLC-UV system with isocratic elution, and the detector was set at 500 nm. The parameters and variables that affect the extraction were investigated. Under optimum conditions: 3% of Triton X-100 (W/V), 10% of Na2CO3 (W/V), heat-assisted at 70 °C for 30 min. Recoveries of the Sudan dyes ranged from 80.70% to 85.45% in chilli powder by CPE method, with all the relative standard deviations of less than 3%. Limit of detection (LOD) and limit of quantification (LOQ) were in the range of 2.0–4.0 and 7.0–12.0 μg kg−1 in chilli powder, respectively.