Development of an enzyme immunoassay for the detection of plant growth regulator inabenfide in rice Original Research Article

A competitive enzyme-linked immunosorbent assay (ELISA) for the plant growth regulator inabenfide was developed using a specific monoclonal antibody (MAB). The effects of rice matrices on the sensibility of ELISA can be removed by adding 0.1% Tween 20. A good reproducibility and accuracy of the proposed ELISA was obtained for rice samples spiked with different amounts of inabenfide. The average recovery for rice samples spiked at 5–500 ppb was 91.6%. Good correlations were obtained between inabenfide measured in rice samples by using the proposed ELISA and that by using a conventional HPLC or GC analysis. This assay can be used in monitoring to determine inabenfide in rice.