Spectral curve deconvolution in micellar systems with H-point curve isolation and H-point standard addition methods Original Research Article

A method for spectral curve deconvolution is described, evaluated and applied to micellar systems. The technique is based on combination of H-point curve isolation method (HPCIM) and H-point standard addition method (HPSAM). HPCIM is used for extracting the spectrum of solute in micellar pseudophase and HPSAM is used for calculation of equilibrium concentrations of solute in aqueous phase for each sample. The outputs of procedure are spectrum of dye molecules in micellar pseudophase free from contribution of the dye in the aqueous phase, and partition coefficients of considered solutes between micelle and water phase. The effects of noise and extent of solute partitioning on the reliability of the method are evaluated using model data. The applications of the method to the study of interaction of two different solutes, m-cresol purple (m-CP) and Azure C with Brij-35 and 2-amino-cyclopentene-1-dithiocarboxylic acid (ACDA) with cetyltrimethylamonium bromide (CTAB) micellar systems are presented.