Determination of primary active constituents in Cannabis preparations by high-resolution gas chromatography/flame ionization detection and high-performance liquid chromatography/UV detection Original Research Article

For a complete quantitative analysis of primary active constituents in Cannabis preparations Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN), we have compared two different chromatographic techniques, high-resolution gas chromatography (HRGC)/flame ionization detection (FID) and high-performance liquid chromatography (HPLC)/UV. The two different methods have been validated using crude drug (hashish) with methyloleate and tetraphenylethylene as internal standard for HRGC/FID and HPLC/UV, respectively.