Use of the diphasic dialysis as a new extraction procedure in the determination of enrofloxacin and ciprofloxacin in egg Original Research Article

We have developed a new method for the analysis of the fluoroquinolones enrofloxacin and ciprofloxacin in eggs using a diphasic dialysis procedure as extraction and purification method. High pressure liquid chromatography–mass spectrometry (HPLC–MS) was used for the confirmatory determination of these compounds. The method was found to be linear between 10 and 800 ng g−1 for enrofloxacin, and between 20 and 1600 ng g−1 for ciprofloxacin. The recovery percentages were in the 70–104% range for enrofloxacin, and 55–97% for ciprofloxacin. The assay described was repeatable and reproducible with a limit of quantitation of 2 and 4 ng g−1 for enrofloxacin and ciprofloxacin from egg, respectively.