Comparison of two methods for the extraction of fat from human milk Original Research Article

We compared two methods for the extraction of fat from human milk. Pure fat extraction techniques are necessary for qualitative and quantitative analysis of milk fat, of which triglycerides account for more than 98%. Method I was a conventional liquid–liquid system for the fat extraction while method II was a faster approach using a haematocrit technique. No significant differences were observed between both methods neither in the fat content determined gravimetrically, nor in qualitative and quantitative analysis of triglycerides by high-performance liquid chromatography (HPLC) with evaporative light-scattering detection (ELSD). We conclude that method II offers substantial advantages over the conventional method (method I). The former requires less reagents and material and is simpler and less time-consuming (approximately 30 min instead of 90 min). Therefore, a new method will make it possible to extract fat of more human milk in the same time.