The ultimate veal calf reference experiment: Hormone residue analysis data obtained by gas and liquid chromatography tandem mass spectrometry Original Research Article

A lifetime controlled reference experiment has been performed using 42 veal calves, 21 males and 21 females which were fed and housed according to European regulations and common veterinary practice. During the experiment feed, water, urine and hair were sampled and feed intake and growth were monitored. Thus for the first time residue analysis data were obtained from guaranteed lifetime-untreated animals. The analysis was focused on the natural hormones estradiol and testosterone and their metabolites, on 17β- and 17α-nortestosterone, on 17β- and 17α-boldenone and androsta-1,4-diene-3,17-dione (ADD), and carried out by gas chromatography tandem mass spectrometry (GC/MS/MS), an estrogen bioassay and liquid chromatography (LC) MS/MS. Feed, water and hair samples were negative for the residues tested. Female calf urines showed occasionally low levels of 17α-estradiol and 17α-testosterone. On one particular sampling day male veal calf urines showed very high levels of 17α-testosterone (up to 1000 ng mL−1), accompanied by lower levels of estrone and 17β-testosterone. Despite these extreme levels of natural testosterone, 17β-boldenone was never detected in the same urine samples; even 17α-boldenone and ADD were only occasionally beyond CCα (maximum levels 2.7 ng mL−1). The data from this unique experiment provide a set of reference values for steroid hormones in calf urine and demonstrate that 17β-boldenone is not a naturally occurring compound in urine samples.