Title of article :
On-line preconcentration and speciation analysis of Cr(III) and Cr(VI) using bakerʹs yeast cells immobilised on controlled pore glass Original Research Article
Author/Authors :
Amauri A. Meneg?rio، نويسنده , , Patricia Smichowski، نويسنده , , Griselda Polla، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Pages :
7
From page :
244
To page :
250
Abstract :
A study was undertaken to evaluate Saccharomyces cerevisiae as a substrate for the biosorption of Cr(III) and Cr(VI) aiming to the selective determination of these species in aqueous solutions. The yeast cells were covalently immobilised on controlled pore glass (CPG), packed in a minicolumn and incorporated in an on-line flow injection system. The effect of chemical and physical variables affecting the biosorption process was tested in order to select the optimal analytical conditions for the Cr retention by S. cerevisiae. Cr(III) was retained by the immobilised cells and Cr(VI) were retained by CPG. The speciation was possible by selective and sequential elution of Cr(III) with 0.05 mol L−1 HCl and 2.0 mol L−1 HNO3 for Cr(VI). The influence of some concomitant ions up to 20 mg L−1 was also tested. Quantitative determinations of Cr were carried out by means of inductively coupled plasma optical emission spectrometry (ICP OES). Preconcentration factors of 12 were achieved for Cr(III) and 5 for Cr(VI) when 1.7 mL of sample were processed reaching detection limits of 0.45 for Cr(III) and 1.5 μg L−1 for Cr(VI). The speciation of inorganic Cr in different kinds of natural waters was performed following the proposed method. Spiked water samples were also analysed and the recoveries were in all cases between 81 and 103%.
Keywords :
Cr(III) , Immobilisation , Controlled pore glass , Cr(VI) , Preconcentration , Speciation analysis , Saccharomyces cerevisiae
Journal title :
Analytica Chimica Acta
Serial Year :
2005
Journal title :
Analytica Chimica Acta
Record number :
1035497
Link To Document :
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