Author/Authors :
Sharafi، Ebrahim نويسنده Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran , , Khayam Nekoei، Seyyed Mojtaba نويسنده Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran , , Fotokian، Mohamad Hossein نويسنده Department of Agricultural Biotechnology, Shahed University, Tehran, Iran , , Davoodi ، Dariush نويسنده Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran , , Hadavand Mirzaei، Hossein نويسنده Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran , , Hasanloo، Tahereh نويسنده ,
Abstract :
Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after 72 h. In the cultures stimulated by zinc nano-oxide, the hypericin and hyperforin production reached to the maximum (7.87 and 217.45 µg/g DW, respectively), which were 3 and 13-fold higher than the control. The amount of hypericin and hyperforin was increased from 2.07 and 16.27 µg/g DW to 11.18 and 195.62 µg/g DW in iron nano-oxide treated cultures. The cell cultures treated with zinc and iron nano-oxides showed increased hyperforin production as compared to the hypericin production. These observations suggested that nano-particles can be appropriate candidates for elicitation studies of in vitro secondary metabolites production.
