Author/Authors :
Abolhasani، Jafar نويسنده Department of Chemistry, Tabriz branch, Islamic Azad University, Tabriz, Iran Abolhasani, Jafar , Motallebpour Sangestany، Elham نويسنده Department of Chemistry, Tabriz branch, Islamic Azad University, Tabriz, Iran. Motallebpour Sangestany, Elham , Vahid، Behrouz نويسنده 2- Department of Chemical Engineering, Tabriz Branch, Islamic Azad University, Tabriz, Iran Vahid, Behrouz
Abstract :
In this study, a sensitive and simple spectrophotometric method has been developed for determination of amoxicillin (AMX) using gold nanoparticles (AuNPs) synthetized by chemical reduction method. This method is based on addition of AMX to AuNPs and nanoparticles aggregation, consequently absorption band of nanoparticles decreased. The optimization of experimental variables was investigated by examining pH, temperature, buffer type and amount, AMX and AuNPs amounts and process time. In the desired condition, the linear calibration graph in the range of 10-250 ?g/l was obtained with proper correlation coefficient (R2=0.99). Limit of detection (LOD) and relative standard deviation (RSD %) were calculated as 0.12 ?g/l and 0.61%, respectively. The proposed method was successfully applied to the AMX measurement in pharmaceutical, real water and biological samples.
