Title of article
Manipulating the biochemical nanoenvironment around single molecules contained within vesicles Original Research Article
Author/Authors
Daniel T. Chiu، نويسنده , , Clyde F. Wilson، نويسنده , , Anders Karlsson، نويسنده , , Anna Danielsson، نويسنده , , Anders Lundqvist، نويسنده , , Anette Str?mberg، نويسنده , , Frida Ryttsén، نويسنده , , Maximilian Davidson، نويسنده , , Arne Rosen and Sture Nordholm، نويسنده , , Owe Orwar، نويسنده , , Richard N. Zare and others، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 1999
Pages
7
From page
133
To page
139
Abstract
A method to study single-molecule reactions confined in a biomimetic container is described. The technique combines rapid vesicle preparation, optical trapping and fluorescence confocal microscopy for performing simultaneous single-vesicle trapping and single-molecule detection experiments. The collisional environment between a single enzyme and substrate inside a vesicle is characterized by a Brownian dynamics Monte Carlo simulation.
Keywords
Biochemical nanoenvironment , Single molecules , Vesicles
Journal title
Chemical Physics
Serial Year
1999
Journal title
Chemical Physics
Record number
1056540
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