Author/Authors :
Daniel T. Chiu، نويسنده , , Clyde F. Wilson، نويسنده , , Anders Karlsson، نويسنده , , Anna Danielsson، نويسنده , , Anders Lundqvist، نويسنده , , Anette Str?mberg، نويسنده , , Frida Ryttsén، نويسنده , , Maximilian Davidson، نويسنده , , Arne Rosen and Sture Nordholm، نويسنده , , Owe Orwar، نويسنده , , Richard N. Zare and others، نويسنده ,
Abstract :
A method to study single-molecule reactions confined in a biomimetic container is described. The technique combines rapid vesicle preparation, optical trapping and fluorescence confocal microscopy for performing simultaneous single-vesicle trapping and single-molecule detection experiments. The collisional environment between a single enzyme and substrate inside a vesicle is characterized by a Brownian dynamics Monte Carlo simulation.
