Immobilization of lysozyme on the layered silicate RUB-15

Adsorption of lysozyme over layered silicate, denoted as RUB-15, and the modified compound RUB-15-nano have been studied. The amount of enzyme adsorbed onto RUB-15 or RUB-15-nano was smaller than that recently reported as being adsorbed onto mesoporous silicas such as MCM-41 or SBA-15. However, lysozyme adsorbed onto RUB-15-nano exhibits enzymatic activity. Lysozyme was found to be immobilized on RUB-15-nano, which was modified from the layered silicate RUB-15. RUB-15-nano may therefore be useful as an inorganic substrate to immobilize enzymes.