The Action of Adenosine Deaminase (E.C. 3.5.4.4.) on Adenosine and Deoxyadenosine Acetates: The Crucial Role of the 5′-Hydroxy Group for the Enzyme Activity

From adenosine 1, 2′-deoxyadenosine 3 and 3′-deoxyadenosine 5 all the acetates were prepared by lipase-catalyzed reactions. Only the acetates with free 5′-hydroxy group were deaminated by adenosine deaminase (ADA), confirming the crucial role of 5′-OH for the enzyme activity.