Title of article :
Cyclodextrin-assisted Glycan Chain Extension on a Protected Glycosyl Amino Acid
Author/Authors :
Gregor Dudziak، نويسنده , , Nicole Bézay، نويسنده , , Tilo Schwientek، نويسنده , , Henrik Clausen، نويسنده , , Horst Kunz، نويسنده , , Andreas Liese، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2000
Abstract :
By the use of cyclodextrins, we have enhanced the solubility of the protected amino acid glycan Fmoc-Thr(GalNAcα1)-OtBu (1b) up to 100-fold. This improvement enabled us to carry out an enzymatic glycosylation employing a β-galactosidase in combination with an α2,3-sialyltransferase without the aid of organic cosolvents. After optimization of the one-pot reaction, the sialylated core 1 structure Fmoc-Thr[Neu5Ac(α2-3)Gal(β1-3)GalNAcα1]-OtBu (3b) could be obtained with 50% yield.
Keywords :
Biosynthesis , Cyclodextrins , Glycopeptides , Glycosidation
Journal title :
Tetrahedron
Journal title :
Tetrahedron
