Towards nucleopeptides containing any trifunctional amino acid (II)

Nucleopeptides with no restriction in the amino acid composition can be synthesized using stepwise solid-phase methodology. Best conditions for the protection of arginine and cysteine, as well as for the final deprotection treatment are established, and our conclusions can be extended to the preparation of any peptide–oligonucleotide hybrid. The association between certain side reactions and nucleopeptide sequences is also discussed.