Oligonucleotide conjugates based on acyclonucleosides and their use in DNA hybridization assays

Synthesis of two oligonucleotide building blocks based on acyclonucleosides (, ) which enable the introduction of several nonluminescent and luminescent lanthanide(III) chelates to the oligonucleotide structure is described. They were used in an instrument-assisted DNA synthesis in a standard manner. A modified deprotection procedure was used to ensure metal complexation. Also the applicability of these oligonucleotide conjugates to DNA hybridization assays is demonstrated.