Author/Authors :
Wang، Xinming نويسنده Department of Chemistry, College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, CHINA , , Xu، Dongling نويسنده Department of Chemistry, College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, CHINA ,
Abstract :
The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in ?-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with ?-cyclodextrin. All the evidences indicated that the binding modes between ?-cyclodextrin-brilliant green and DNA were grooving binding and partial non-classical intercalative binding. The binding ratio of the inclusion complex with DNA is 6:1. The calculated thermodynamic parameters suggested that the binding of the inclusion complex to DNA was driven mainly by entropy.
