Studies on the biosynthesis of secobotryane skeleton

The labelling and coupling patterns of secobotrytrienediol, biosynthesised from [1-13C] and [1,2-13C2]-acetate by the fungus Botrytis cinerea, have been used to define the mode of formation and the biogenetic origin of secobotrytrienediol. [10-2H]-Botrydiol was not incorporated into the secobotryane skeleton. In addition, this feeding experiment led to the isolation of three new unlabelled derivatives possessing a secobotryane skeleton, secobotrydiene-3,10,15-triol, secobotrydiene-3,4,10,15-tetraol, and secobotrytriene-10,12,15-triol.