Title of article :
Preparation of highly deuterated zeaxanthin, lycopene, and β-carotene from fully deuterated mevalonate using engineered Escherichia coli
Author/Authors :
Tadashi Eguchi، نويسنده , , Yasumasa Dekishima، نويسنده , , Hideki Aizawa، نويسنده , , Hideyuki Tamegai، نويسنده , , Katsumi Kakinuma، نويسنده , , Norihiko Misawa، نويسنده , , Tomohisa Kuzuyama، نويسنده , , Haruo Seto، نويسنده ,
Abstract :
Carotenoids are important natural pigments produced by various microorganisms and plants. Specific deuterium-labeling of these compounds is invaluable in biochemical and physiochemical research. In this paper, preparation of highly deuterated zeaxanthin, lycopene, and β-carotene using engineered Escherichia coli with fully deuterated mevalonate is described. Also described are physico-chemical properties of the obtained deuterated carotenoids.
Keywords :
Isotope labeling , Carotenoids , Engineered Escherichia coli , Biosynthesis
