Nicotinamide-independent asymmetric bioreduction of Cdouble bond; length as m-dashC-bonds via disproportionation of enones catalyzed by enoate reductases

The asymmetric bioreduction of activated Cdouble bond; length as m-dashC-bonds catalyzed by a single flavoprotein was achieved via direct hydrogen transfer from a sacrificial 2-enone or 1,4-dione as hydrogen donor without requirement of a nicotinamide cofactor. Due to its simplicity, this system has clear advantages over conventional FAD-recycling systems.