Author/Authors :
Saihui Zhang، نويسنده , , Liang Han، نويسنده , , Chuan-guang Li، نويسنده , , Jun Wang، نويسنده , , Wei Wang، نويسنده , , Zhi Yuan، نويسنده , , Xueping Gao، نويسنده ,
Abstract :
In this study, a receptor for phosphorylated peptide was developed. The receptor contains Zn2+–dipicolylamine (Zn–Dpa) moiety at one end and guanidiniocarbonyl pyrrole at the other. The association between the receptor and CTD (carboxy-terminal domain of RNA polymerase II) peptides with different phosphorylated patterns was investigated by isothermal titration calorimetry and molecular docking, and the receptor showed much higher affinity towards bis-phosphorylated CTD peptide with affinity constant K=1.09×105.
Keywords :
Artificial receptor , Phosphorylated peptides , CTD peptides , Isothermal titration calorimetry
