Author/Authors :
Rashidi، Mohammad Reza نويسنده , , Eisa-Khaje، Jalal نويسنده Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran , , Farzadi، Laya نويسنده Department of Obstetrics and Gynecology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , , Darabi، Masoud نويسنده Department of Biochemistry and Clinical Laboratories, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , , Gasemzadeh، Alieh نويسنده Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran , , Shahnazi، Vahideh نويسنده , , Fayezi، Shabnam نويسنده Students Research Committee, Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Mehdizadeh، Amir Hossein نويسنده Department of Management, Islamic Azad University, South Tehran Branch, Tehran, Iran , , Haji-Hosseini ، Reza نويسنده Haji-Hosseini , Reza , Nouri، Mohammad نويسنده ,
Abstract :
Background: Paraoxonase-3 (PON3), as a high density lipoprotein (HDL)-associated lactonase,
is capable of preventing the oxidative modification of low density lipoprotein (LDL).
PON3 activity in follicular fluid (FF) is three times more than its activity in serum. However,
the detailed role of PON3 in women’s fertility remains unknown. The aim of this study was to
investigate the correlation between PON3 activity in the FF of women undergoing assisted reproductive
technique (ART), in vitro fertilization (IVF), or intra-cytoplasmic sperm injection (ICSI).
Materials and Methods: This cross-sectional study consisted of 50 women from couples
with male factor infertility (MFI) or with female factor infertility (FFI). The FF samples were
obtained during the ART intervention. PON3 activity, HDL cholesterol (HDL C), total antioxidant
status (TAS) and the level of malondialdehyde (MDA) were determined. The morphology
of the embryo was determined using embryo cell number (ECN) and embryo fragmentation
score (EFS). In addition, fertilization rate (FR) was used an oocyte fertilization index.
Results: Of 50 women, 20 women belonged to FFI group and the remaining 30 women
belonged to MFI group. PON3 activity in FF of women in FFI group was significantly
lower (p < 0.05) in comparison with corresponding value in MFI group. The value of
PON3 activity/MDA in the FFI group was lower than that in MFI group. Moreover,
MDA level in the FF of FFI group was significantly higher (p < 0.05) than its concentration
in MFI group. Meanwhile, no significant difference was found in HDL-C concentration
and TAS of both groups. No significant correlation was observed between the ECN
and FF biochemical parameters. There was also a negative correlation between FR and
MDA (r=-0.42, p=0.02), whereas a positive relation between FR with PON3 activity
(r=0.59, p=0.004), HDL-C (r=0.35, p=0.04) and PON3/MDA (r=0.59, p=0.001).
Conclusion: According to the results of this study, PON3 activity level as a key component
of antioxidant system in FF may directly be associated with the success rate of ART
and fertilization rate in women.
