Author/Authors :
Sereshki، Nasrin نويسنده Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , , Gharagozloo، Marjan نويسنده Departments of Immunology, Isfahan University of Medical Sciences, Isfahan , , Ostadi، Vajihe نويسنده Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , , Ghahiri، Ataollah نويسنده , , ROGHAEI، MOHAMMAD ALI نويسنده , , Mehrabian، Ferdos نويسنده Department of Obstetrics and Gynecology, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran , , Andalib، Alireza نويسنده , , Hassanzadeh، Akbar نويسنده Derpartment of Food Technology, Food Security Research Center, Isfahan University of Medical Science, Isfahan , , Hosseini، Habibolla نويسنده Isfahan University of Medical Sciences, Isfahan, Iran , , REZAEI، ABBAS ALI نويسنده ,
Abstract :
Background: Disorders in immune system regulation may result in pregnancy abnormalities
such as recurrent spontaneous abortion (RSA). This study aims to determine
the ratio of regulatory T (Treg) and T helper (Th) 17 cells in unexplained RSA (URSA)
women during proliferative and secretory phases of their menstrual cycles compared to
healthy non-pregnant women.
Materials and Methods: In this case control study, 25 women with URSA and 35 healthy,
non-pregnant women were enrolled. The percentage of Th17 and Treg cells in participants
peripheral blood were determined by flow cytometry.
Results: The percentage of Th17 cells and their related cytokines in serum (IL-17A)
were higher in the proliferative and secretory phases of the menstrual cycles of URSA
women compared to the control women. However, a lower percentage of Treg cells and
their related cytokines in serum, transforming growth factor (TGF) B1 and interleukin
(IL)-10 were detected in the proliferative but not the secretory phase of the URSA group.
The ratio of Th17/CD4+ Treg was higher in the URSA group than the control group. We
observed an increased ratio of Th17/CD4+ Treg during the proliferative and secretory
phases in URSA women.
Conclusion: The imbalance between Th17 and Treg cells during the proliferative
phase of menstrual cycles in the URSA group may be considered a cause for spontaneous
abortion.
