Author/Authors :
Akbary، Paria نويسنده Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, College of Agriculture and Natural Resources, University of Tehran, , , Mirvaghefi، Alireza نويسنده Department of Environmental Science, Faculty of Natural Resources, Isfahan University of Technology, Isfahan, Iran, P.O. Box 8415683111, Isfahan, Iran , , Akhlaghi، Mostafa نويسنده Department of Aquatic Animal Health Unit, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Mojazi Amiri ، Bagher نويسنده , , Fereidouni، Mohammad Saeid نويسنده Aquatic Animal Health Unit, School of Veterinary Medicine, Shiraz University, Shiraz, Iran, Po Box 71345-173. ,
Abstract :
Letrozole is a synthetic aromatase inhibitor and interfere in the committed step in the synthesis of endogenous estrogens from androgens. Also estrogens regulate the immune system in teleost. Changes of 17- B- esrtradiol (E2), serum immunoglobulin and lysozyme levels were measured using a method based on the ability of lysozyme to lyse the bacterium Micrococcus lysodeikticus, enzyme-linked immunosorbent assay (ELISA) and ELISA respectively. Twelve broodstocks were injected weekly with 2.5 mg kg-1 letrozole (an endocrine disrupter component) two months before spawning season and vaccinated intraperitoneally (i.p) with a bacterin (inactivated L. garviae) one month before spawning. Twelve broodstocks for vaccination and twelve female rainbow trout as control group were also immiunised (i.p) with the bacterin and injected (i.p) with PBS, respectively. In the group received 2.5 mg AI kg-1 per week, serum E2 levels were significantly lower than that of other groups. Total immunoglobulin level and lysozyme activity were significantly higher in the parents received 2.5 mg kg-1 per week and were immunized with 10-9 cells ml-1 Lactococcus garvieae compared to the group which immunized with L. garvieae and the control (non- immunized). The present study, suggests that aromatase inhibitors such as letrozole may be a potential tool to regulate the synthesis of E2, is involved in the hormone- immune system interaction in rainbow trout.
