Title of article :
Identification and Characterization of a Ca2+-Dependent Actin Filament-Severing Protein from Lily Pollen
Author/Authors :
Staiger، Christopher J. نويسنده , , Fan، Xiaoxue نويسنده , , Hou، Jian نويسنده , , Chen، Xiaoliang نويسنده , , Chaudhry، Faisal نويسنده , , Ren، Haiyun نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
-3978
From page :
3979
To page :
0
Abstract :
It is well known that a tip-focused intracellular Ca^2+ gradient and the meshwork of short actin filaments at the tip region are necessary for pollen tube growth. However, little is known about the connections between the two factors. Here, a novel Ca^2+-dependent actin-binding protein with molecular mass of 41 kD from lily (Lilium davidii) pollen (LdABP41) was isolated and purified with DNase I chromatography. Our purification procedure yielded about 0.6 mg of LdABP41 with >98% purity from 10 g of lily pollen. At least two isoforms with isoelectric points of 5.8 and 6.0 were detected on two-dimensional gels. The results of N-terminal sequencing and mass-spectrometry analysis of LdABP41 showed that both isoforms shared substantial similarity with trumpet lily (Lilium longiflorum) villin and other members of the gelsolin superfamily. Negative-stained electron microscope images showed that LdABP41 severed in vitro-polymerized lily pollen F-actin into short actin filaments in a Ca^2+-sensitive manner. Microinjection of the anti-LdABP41 antibody into germinated lily pollen demonstrated that the protein was required for pollen tube growth. The results of immunolocalization of the protein showed that it existed in the cytoplasm of the pollen tube, especially focused in the tip region. Our results suggest that LdABP41 belongs to the gelsolin superfamily and may play an important role in controlling actin organization in the pollen tube tip by responding to the oscillatory, tipfocused Ca^2+ gradient.
Keywords :
Auditory stimulation , Dolphins , Transcranial magnetic stimulation , Consciousness , Nonlocality , Patterned photostimulation , Visual evoked potential , Transferred potential , Chimpanzees
Journal title :
PLANT PHYSIOLOGY
Serial Year :
2004
Journal title :
PLANT PHYSIOLOGY
Record number :
113847
Link To Document :
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