Title of article :
Characterization of AmpC, CTX-M and MBLs types of B-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum B-lactamases in Kerman, Iran
Author/Authors :
Mansouri، Shahla نويسنده , , Kalantar Neyestanaki، Davood نويسنده Department of Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, IR Iran , , Shokoohi، Mostafa نويسنده Physiology Research Center, Regional Knowledge Hub for HIV/AIDS Surveillance, Kerman , , Halimi، Shahnaz نويسنده Department of Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, IR Iran , , Beigverdi، Reza نويسنده Department of Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, IR Iran , , Rezagholezadeh، Fereshteh نويسنده Department of Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, IR Iran , , Hashemi، Ali نويسنده Shahid Beheshti University of Medical Sciences ,
Abstract :
Background:: Extended spectrum B-lactamases (ESBLs) and AmpC B-lactamases enzyme are major sources of resistance to B-lactam antibiotics especially in Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae. Increasing frequency of the co-existence of ESBLs with AmpC-B-lactamases in bacteria is a serious threat for treating bacterial infections.
Objectives:: The aim of this study was to determine the presence of AmpC and CTX-M types of B-lactamases in clinical isolates of E. coli and K. pneumoniae producing ESBLs.
Materials and Methods:: Resistance to different antibiotics was determined using the standard disk diffusion method. ESBLs, MBLs and AmpC-B-lactamases were detected by the combination double disk test (CDDT) method and polymerase chain reaction (PCR) was used to determine blaCTX-M genes in the ESBLs and AmpC positive isolates.
Results:: The prevalence of ESBLs and AmpC-B-lactamase producer isolates was 181 (43.8%) and 133 (37.2%), respectively. The prevalence of blaCTX-M among isolates was 61 (14.7%).
Conclusions:: Outbreak of isolates co-expressing AmpC-B-lactamases and ESBLs can cause serious problems in the future, regarding the treatment of infections caused by these common enteric pathogens.
