Title of article :
DNA Polymerase Template Interactions Probed by Degenerate Isosteric Nucleobase Analogs Original Research Article
Author/Authors :
Gregory M. Watt، نويسنده , , John Lund، نويسنده , , Michaela Levens، نويسنده , , V.S. Kumar Kolli، نويسنده , , Royston Jefferis، نويسنده , , Geert-Jan Boons، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2003
Pages :
11
From page :
815
To page :
825
Abstract :
The development of novel artificial nucleobases and detailed X-ray crystal structures for primer/template/DNA polymerase complexes provide opportunities to assess DNA-protein interactions that dictate specificity. Recent results have shown that base pair shape recognition in the context of DNA polymerase must be considered a significant component. The isosteric azole carboxamide nucleobases (compounds 1–5; ) differ only in the number and placement of nitrogen atoms within a common shape and therefore present unique electronic distributions that are shown to dictate the selectivity of template-directed nucleotide incorporation by DNA polymerases. The results demonstrate how nucleoside triphosphate substrate selection by DNA polymerase is a complex phenomenon involving electrostatic interactions in addition to hydrogen bonding and shape recognition. These azole nucleobase analogs offer unique molecular tools for probing nonbonded interactions dictating substrate selection and fidelity of DNA polymerases.
Journal title :
Chemistry and Biology
Serial Year :
2003
Journal title :
Chemistry and Biology
Record number :
1158693
Link To Document :
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