Author/Authors :
Gaetano Castaldo، نويسنده , , Jurica Zucko، نويسنده , , Sibylle Heidelberger، نويسنده , , Du?ica Vujaklija، نويسنده , , Daslav Hranueli، نويسنده , , John Cullum، نويسنده , , Pakorn Wattana-Amorn، نويسنده , , Matthew P. Crump، نويسنده , , John Crosby and Matthew P. Crump، نويسنده , , Paul F. Long، نويسنده ,
Abstract :
Aklanonic acid is synthesized by a type II polyketide synthase (PKS) composed of eight protein subunits. The network of protein interactions within this complex was investigated using a yeast two-hybrid system, by coaffinity chromatography and by two different computer-aided protein docking simulations. Results suggest that the ketosynthase (KS) α and β subunits interact with each other, and that the KSα subunit also probably interacts with a malonyl-CoA:ACP acyltransferase (DpsD), forming a putative minimal synthase. We speculate that DpsD may physically inhibit the priming reaction, allowing the choice of propionate rather than acetate as the starter unit. We also suggest a structural role for the cyclase (DpsY) in maintaining the overall structural integrity of the complex.
