Author/Authors :
Abel Rosado، نويسنده , , Glenn R. Hicks، نويسنده , , Lorena Norambuena، نويسنده , , Ilana Rogachev، نويسنده , , Sagit Meir، نويسنده , , Lucille Pourcel، نويسنده , , Jan Zouhar، نويسنده , , Michelle Q. Brown، نويسنده , , Marietta P. Boirsdore، نويسنده , , Rachel S. Puckrin، نويسنده , , Sean R. Cutler، نويسنده , , Enrique Rojo، نويسنده , , Asaph Aharoni، نويسنده , , Natasha V. Raikhel، نويسنده ,
Abstract :
Sortin1 is a chemical genetic-hit molecule that causes specific mislocalization of plant and yeast-soluble and membrane vacuolar markers. To better understand its mode of action, we designed a Sortin1-hypersensitive screen and identified several Sortin1-hypersensitive and flavonoid-defective mutants. Mechanistically, Sortin1 mimics the effect of the glutathione inhibitor buthionine sulfoximine and alters the vacuolar accumulation of flavonoids, likely blocking their transport through vacuole-localized ABC transporters. Structure-activity relationship studies conducted in Arabidopsis revealed the structural requirements for Sortin1 bioactivity and demonstrated that overlapping Sortin1 substructures can be used to discriminate between vacuolar-flavonoid accumulations and vacuolar-biogenesis defects. We conclude that Sortin1 is a valuable probe for dissecting novel links among flavonoid transport, vacuolar integrity, and the trafficking of vacuolar targeted cargoes in Arabidopsis.
